Hyaluronic acid (HA) is a natural polymer and a key component of the extracellular matrix (ECM), playing a crucial role in wound healing and tissue repair due to its unique properties and physiological functions. This review highlights the use of HA in wound healing, particularly in hard-to-heal wounds, and its applications in ophthalmology and otorhinolaryngology. HA maintains a moist environment, stimulates growth factors, and promotes cell migration, making it an effective agent in various medical fields. The review covers the biological activities of HA, its production methods, and its use in different forms of wound dressings, including gauze, hydrogels, films, sponges, and scaffolds. It also discusses the combination of HA with bioactive agents to enhance therapeutic outcomes. Additionally, the review explores HA's role in ophthalmology, such as in corneal collagen cross-linking and contact lens solutions, and in otorhinolaryngology, including its use in treating tympanic membrane perforations and oral mucositis. Despite the promising results, more randomized studies are needed to establish guidelines for the use of HA in wound management.Hyaluronic acid (HA) is a natural polymer and a key component of the extracellular matrix (ECM), playing a crucial role in wound healing and tissue repair due to its unique properties and physiological functions. This review highlights the use of HA in wound healing, particularly in hard-to-heal wounds, and its applications in ophthalmology and otorhinolaryngology. HA maintains a moist environment, stimulates growth factors, and promotes cell migration, making it an effective agent in various medical fields. The review covers the biological activities of HA, its production methods, and its use in different forms of wound dressings, including gauze, hydrogels, films, sponges, and scaffolds. It also discusses the combination of HA with bioactive agents to enhance therapeutic outcomes. Additionally, the review explores HA's role in ophthalmology, such as in corneal collagen cross-linking and contact lens solutions, and in otorhinolaryngology, including its use in treating tympanic membrane perforations and oral mucositis. Despite the promising results, more randomized studies are needed to establish guidelines for the use of HA in wound management.