The study investigates the role of YTHDF3, a cytoplasmic m6A-binding protein, in the regulation of mRNA metabolism. YTHDF3 is shown to facilitate protein synthesis in synergy with YTHDF1 and affect mRNA decay mediated by YTHDF2. YTHDF3 binds m6A-modified mRNAs and shares common targets with YTHDF1 and YTHDF2. Depletion of YTHDF3 reduces translation efficiency of its targets and affects the RNA-binding specificity of YTHDF1 and YTHDF2. YTHDF3 functions as a hub for partitioning m6A-modified transcripts among the three proteins, potentially affecting mRNA stability and translation. The results suggest that YTHDF3 plays a critical role in coordinating the metabolism of m6A-modified mRNAs in the cytoplasm.The study investigates the role of YTHDF3, a cytoplasmic m6A-binding protein, in the regulation of mRNA metabolism. YTHDF3 is shown to facilitate protein synthesis in synergy with YTHDF1 and affect mRNA decay mediated by YTHDF2. YTHDF3 binds m6A-modified mRNAs and shares common targets with YTHDF1 and YTHDF2. Depletion of YTHDF3 reduces translation efficiency of its targets and affects the RNA-binding specificity of YTHDF1 and YTHDF2. YTHDF3 functions as a hub for partitioning m6A-modified transcripts among the three proteins, potentially affecting mRNA stability and translation. The results suggest that YTHDF3 plays a critical role in coordinating the metabolism of m6A-modified mRNAs in the cytoplasm.