dbCAN2 is an updated meta server for automated carbohydrate-active enzyme (CAZyme) annotation, integrating three state-of-the-art tools: HMMER, DIAMOND, and Hotpep. The server accepts both nucleotide and protein sequences, and can identify transcription factors (TFs), transporters (TCs), and CAZyme gene clusters (CGCs). It combines the outputs of the three tools to improve annotation accuracy and offers visualization options, including Venn diagrams and detailed results. dbCAN2 also provides a web interface for users to submit queries and access results, making it a valuable resource for researchers in bioinformatics and microbiology. The server is updated annually to incorporate the latest databases and tools, ensuring its relevance and utility in the field.dbCAN2 is an updated meta server for automated carbohydrate-active enzyme (CAZyme) annotation, integrating three state-of-the-art tools: HMMER, DIAMOND, and Hotpep. The server accepts both nucleotide and protein sequences, and can identify transcription factors (TFs), transporters (TCs), and CAZyme gene clusters (CGCs). It combines the outputs of the three tools to improve annotation accuracy and offers visualization options, including Venn diagrams and detailed results. dbCAN2 also provides a web interface for users to submit queries and access results, making it a valuable resource for researchers in bioinformatics and microbiology. The server is updated annually to incorporate the latest databases and tools, ensuring its relevance and utility in the field.