miRWalk2.0 is an advanced and comprehensive resource for microRNA (miRNA)-target interactions, offering the largest collection of predicted and experimentally verified interactions (~949 million). It integrates 13 prediction datasets and provides a multilayered view of data, including miRNAs, genes, epigenomics, pathways, ontologies, protein classes, phenotypes, genotypes, single-nucleotide polymorphisms, functional networks, tandem mass spectra, and relevant PubMed articles. The web interface includes a predicted-target module and a validated-target module, allowing users to download information on human homologous genes across 15 species for interspecies analysis. miRWalk2.0 also features a "Customized data set" function for large-scale enrichment analysis and a platform for comparing miRNA-lncRNA interactions. It supports genomic location searches to identify miRNAs coexpressed with host or neighboring genes, aiding in the identification of miRNAs involved in specific conditions. The resource is updated regularly, with the validated-target module updated every 3 months and the predicted-target module updated twice a year.miRWalk2.0 is an advanced and comprehensive resource for microRNA (miRNA)-target interactions, offering the largest collection of predicted and experimentally verified interactions (~949 million). It integrates 13 prediction datasets and provides a multilayered view of data, including miRNAs, genes, epigenomics, pathways, ontologies, protein classes, phenotypes, genotypes, single-nucleotide polymorphisms, functional networks, tandem mass spectra, and relevant PubMed articles. The web interface includes a predicted-target module and a validated-target module, allowing users to download information on human homologous genes across 15 species for interspecies analysis. miRWalk2.0 also features a "Customized data set" function for large-scale enrichment analysis and a platform for comparing miRNA-lncRNA interactions. It supports genomic location searches to identify miRNAs coexpressed with host or neighboring genes, aiding in the identification of miRNAs involved in specific conditions. The resource is updated regularly, with the validated-target module updated every 3 months and the predicted-target module updated twice a year.