The study demonstrates the successful and efficient reprogramming of murine and human embryonic fibroblasts to induced pluripotent stem (iPS) cells using piggyBac (PB) transposition. The PB transposon/transposase system, which requires only inverted terminal repeats and transient expression of the transposase enzyme, was used to deliver doxycycline-inducible transcription factors (c-Myc, Klf4, Oct4, and Sox2). The resulting iPS cells expressed key pluripotency markers and successfully differentiated into various adult cell types in rigorous assays. The authors also showed that the individual P insertions could be removed from established iPS cell lines, providing a valuable tool for further research. Additionally, they demonstrated the traceless removal of reprogramming factors joined with 2A sequences. The study highlights the advantages of PB transposition, including technical simplification, improved accessibility, and the ability to produce xeno-free human iPS cells.The study demonstrates the successful and efficient reprogramming of murine and human embryonic fibroblasts to induced pluripotent stem (iPS) cells using piggyBac (PB) transposition. The PB transposon/transposase system, which requires only inverted terminal repeats and transient expression of the transposase enzyme, was used to deliver doxycycline-inducible transcription factors (c-Myc, Klf4, Oct4, and Sox2). The resulting iPS cells expressed key pluripotency markers and successfully differentiated into various adult cell types in rigorous assays. The authors also showed that the individual P insertions could be removed from established iPS cell lines, providing a valuable tool for further research. Additionally, they demonstrated the traceless removal of reprogramming factors joined with 2A sequences. The study highlights the advantages of PB transposition, including technical simplification, improved accessibility, and the ability to produce xeno-free human iPS cells.